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Metabolism of Bovine Semen. XIX. Products of Fructose Metabolism by Washed Spermatozoa ¹

Department of Dairy Science, The Pennsylvania State University, University Park 16802

ABSTRACT

The production of ¹⁴C0₂ by washed bovine spermatozoa was determined in Krebs-Hensleit Ringer and in phosphate buffers with labeled fructose, glucose, and pyruvate as substrates. The ¹⁴C0₂ output from fructose and glucose was greater in Ringer, but for pyruvate it was greater in phosphate. With adenosine triphosphate in the Ringer buffer, fructose-6-phosphate, glucose-6-phosphate, and phosphoenolpyruvate were identifled as products of fructose-¹⁴C metabolism. Metabolic traps of unlabeled triose phosphates produced some increase in the accumulation of radioactive phosphate esters but did not result in the identification of additional intermediates. No differences between fructose-l-¹⁴C and fructose-6-¹⁴C were noted in the yield of ¹⁴C0₂ or in the identified nonvolatile products which included asparate, glutamate, lactate, citrate, fumarate, malate, and succinate. These data provide no reason to suspect deviation from the Embden-Meyerhof pathway and the citric acid cycle in utilization of fructose by bovine spermatozoa.

¹ Authorized for publication on January 14, 1969, as Paper no. 3530 in the journal series of the Pennsylvania Agricultural Experiment Station. Supported in part by U.S. Atomic Energy Commission Contract AT (30-1)-1849.

² The data contained in this paper are part of a thesis submitted by the junior author to the Graduate School of The Pennsylvania State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy.