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Metabolism of Bovine Semen. XVIII. Effect of Keto Acids on Glutamate Metabolism¹

Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

Washed bovine spermatozoa were incubated with glutamate-1- and -5-¹⁴C in the presence of unlabeled pyruvate or -ketoglutarate. In air, both pyruvate and -ketoglutarate depressed ¹⁴CO₂ production from glutamate-1-¹⁴C. Under anoxia, pyruvate tended to stimulate ¹⁴CO₂ production while -ketoglutarate depressed it. In other studies with uniformly labeled glutamate, ¹⁴CO₂ production decreased while oxygen uptake increased as the -ketoglutarate concentration was increased to 10 mM; 20 mM -ketoglutarate inhibited both oxygen uptake and ¹⁴CO₂ production. As unlabeled oxalacetate concentration was increased to 10 mM, the ¹⁴CO₂ production from glutamate-U-¹⁴C was only slightly depressed while oxygen uptake increased. Thus, glutamate degradation proceeded mainly via -ketoglutarate and the citric acid cycle, but keto acid concentration influenced the pathway utilized.

¹ Authorized for publication as Paper no. 3482 in the Journal Series of The Pennsylvania Agricultural Experiment Station. Supported in part by Public Health Service Research Grant no. HD-00039-05.