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Resazurin Test Paper Method for Determining the Sanitary Quality of Raw Milk

Laboratory Section, City Milk and Ice Cream Production Division, Snow Brand Dairy Industry Co. Ltd., Fukuro-Cho, Kita-ku, Tokyo, Japan
Faculty of Agriculture, Okayama University, Okayama, Japan

ABSTRACT

A new reduction method using resazurin-impregnated filter paper was applied for determining the sanitary quality of raw milk. Experimental results of standard agar plate and resazurin test paper methods showed a high correlation between the plate count and the resazurin reduction time. By measuring the velocity constant of the reaction, color change from resazurin to resorufin on the filter paper indicated it to be a unimolecular reaction. It was concluded that the resazurin test paper method has the advantage of accuracy and simplicity for a field test.