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Sephadex Equilibrium-Diffusion Technique for Fractionating Whey and Skimmilk Systems¹

Department of Food Science and Industries, University of Minnesota, St. Paul 55101

ABSTRACT

An equilibrium-diffusion process was developed which utilizes Sephadex G-25 for removing low molecular weight solute materials from whey and skimmilk systems. Two successive treatments by the process removed a total of about 67% of the minerals and about 51% of the lactose from acid whey and about 36% of the minerals and about 72% of the lactose from concentrated skimmilk. Equilibration of the low molecular weight constituents between the milk serum phase and the interior of the Sephadex beads was complete within 1 min or less. The extent of fractionation achieved is primarily a function of the mixing ratio of product and Sephadex and the number of successive contact treatments used. The equilibrium-diffusion technique may be useful for developing large-scale, continuous processes for fractionating whey and skimmilk systems into low molecular weight and colloidal constituents.

¹ Scientific Journal Series Paper no. 6946, Minnesota Agricultural Experiment Station.