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Journal of Dairy Science Vol. 51 No. 9 1420-1422
© 1968 by American Dairy Science Association ®
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Active Transport of Glycine by Rumen Epithelium of Betal Goats

Dewan Chand, S. D. Varma and R. P. S. Kushwaha

Department of Biochemistry, Punjab Agricultural University, Hissar (Haryana), India

ABSTRACT

Glycine transport by in vitro preparation of rumen epithelium of betal goats was studied. Transport of glycine across the rumen epithelium and its accumulation inside cell took place against the chemical gradient suggestive of active transport, involving cellular metabolism. The participation of cellular metabolism in this process was also conceived from the observed inhibitory effect on the process by 2,4-dinitrophenol and iodoacetate, the common metabolic inhibitors. The incubation medium was Krebs Ringer-bicarbonate saline alone or containing 50 µM of 2,4-dinitrophenol or 16.1 mM of iodoacetic acid.






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