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Survival of Bull and Ram Spermatozoa in Preparations from Skimmilk

Department of Animal Science, Cornell University, Ithaca, New York

ABSTRACT

Media containing the nondialyzable components of skimmilk were prepared in dialysis sacks by placing the sacks filled with skimmilk in buffered saline and storing at 5 C for 48 hours. Similarly, media containing the dialyzable components of milk were prepared by placing dialysis sacks of buffered saline in skimmilk. When these preparations (alone and in combination) were mixed 1:1 with a solution of 185 mM lactose and electrolytes, bull and ram spermatozoa survived at 37 C better in the nondialyzable than in the dialyzable preparations, and either of these preparations was better than a 1:1 mixture of both. Heating the dialyzable preparation or mixtures of both preparations before use increased survival rates, but heating rendered the lactose solution harmful to bull and ram spermatozoa.

When the concentration of sodium chloride was varied in buffered saline diluents containing heated dialyzable and unheated and heated nondialyzable milk preparations, it was found that media containing the nondialyzable preparations had a higher tonicity (measured by the survival of bull spermatozoa) than the dialyzable preparations. Heating the nondialyzable preparations improved the survival of spermatozoa. Both preparations, either heated or unheated, were lyophilized and used in a factorial experiment at concentrations of 0.5 and 1.0% w/v in media of relative tonicities 0.8 and 1.0. Scores of survival of spermatozoa made after incubation at 30 C for eight hours were lowered by increasing the concentration of the lyophilized preparations and increasing the tonicity of the diluents. However, mean scores of per cent motile spermatozoa in samples from media containing unheated dialyzable and nondialyzable preparations were 25.4 and 24.6, respectively; for media containing preparations heated before lyophilization, the means were 18.8 and 33.8.