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In Vitro Analyses of the Binding of ¹³¹I-Iodide to Milk Protein¹

Bio-Medical Division, Lawrence Radiation Laboratory, University of California, Livermore

ABSTRACT

The experiments reported here were carried out to study the in vitro binding of ¹³¹I to bovine milk protein and the factors influencing this binding. Incubation of inorganic carrier-free ¹³¹I-iodide with fresh raw milk at 37 C for two hours showed as much as 50% of the ¹³¹I bound to milk protein, and that as much as 80–90% could be bound in 12–24 hours. It was also shown that this reaction is both time- and temperature-dependent and could be readily blocked by incubation at 8 C, boiling the milk prior to incubation, addition of carrier iodide or antithyroid agents such as Tapazole (methyl mercaptan imidazole).

Paper chromatography of enzymatic digests of the milk showed the protein-bound ¹³¹I in milk is present as ¹³¹I-labeled monoiodotyrosine. No other ¹³¹I-labeled compounds were observed. These studies would tend to indicate that the binding of ¹³¹I-iodide to milk protein involves an enzymatic oxidation of the iodide.

¹ This work was performed under auspices of the U.S. Atomic Energy Commission.