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Journal of Dairy Science Vol. 51 No. 8 1155-1160
© 1968 by American Dairy Science Association ®
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Comparison of Column and Centrifugal Sephadex Methods for Fractionating Whey and Skimmilk Systems1

C. V. Morr, S. T. Coulter and R. Jenness

Department of Food Science and Industries, University of Minnesota, St. Paul

ABSTRACT

Normal and concentrated whey and skimmilk systems were fractionated into a colloid and a low molecular weight fraction by column and centrifugal procedures using Sephadex G-25 and G-50 Sephadex, and the resulting fractions were analyzed to determine the fractionation efficiency of the process and the composition of the resulting colloid fraction.

Sephadex G-50 columns were more effective than Sephadex G-25 columns for removing lactose and mineral constituents from the concentrated skimmilk colloid fraction. The fractionation efficiency of the centrifugal Sephadex method (Sephadex G-25) was intermediate to that of Sephadex G-25 and G-50 columns. Approximately 90–92% of the total proteins of concentrated whey was recovered in the colloid fraction from the Sephadex G-25 column process compared to ~ 90–95% recovery from concentrated skimmilk. The colloid fractions obtained from concentrated whey and skimmilk samples by the Sephadex G-25 column process exhibited ~ a six-fold and a two-fold enrichment of the protein content, respectively. Fractionation results were somewhat similar for the Sephadex G-25 centrifugal and column methods; however, the centrifugal method permits larger sample volumes with less dilution of the colloid fraction than does the column method.

FOOTNOTES

1 Scientific Journal Series Paper no. 6448, Minnesota Agricultural Experiment Station.






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Copyright © 1968 by the American Dairy Science Association ®.