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Photometric Determination of Fat in Milk

Veksebo Pr. Fredensborg, Denmark

ABSTRACT

Walstra has published (2) some critical remarks concerning my photometrical method for determining fat in milk. He has attempted to repeat my measurements (1) but has changed the procedure on two important points, which makes a comparison of the two methods without quantitative value.

Walstra (2) diluted milk to 0.5% and used a 5-mm optical cell. In my procedure milk is diluted to about 0.2% and the optical cell is only 0.5 mm thick. High dilution and a thin cell are used to eliminate disturbing multiple light scattering. This is best demonstrated on the calibration curve which is linear only under the stated conditions. With milk of high fat content the curve levels off. Therefore, Walstra's conclusions are not valid.