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Effect of 2-Deoxyglucose on Bovine Spermatozoan Metabolism and Preservation

Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

Bovine spermatozoa were used to test the effectiveness of the glucose analog, 2-deoxyglucose (2DG), as a reversible inhibitor of spermatozoan glycolysis, with its possible use as a spermatozoan preservative under practical storage conditions. The results showed that 2DG was an effective inhibitor of spermatozoan glycolysis, with fructolysis being more sensitive to the inhibitory action of 2DG than glucolysis. This inhibition was reversed by adding glucose. The oxidative metabolism of pyruvate and lactate was not inhibited by concentrations of 2DG which completely inhibited the oxidation of fructose. The oxidation of glucose-1-, glucose-6-, and fructose-6-phosphates was inhibited by 2DG. Thus it appears that the analog inhibits at several enzyme steps in the Embden-Meyerhof pathway of glycolysis, but the primary inhibition appears to be on the hexokinase enzyme for fructose. During longer storage, when motility was inhibited and recovered by addition of glucose, recovery became more difficult with longer storage. Thus the addition of 2DG appeared detrimental to livability of the cells under the conditions employed and consequently cannot be used as a metabolic control mechanism for preserving spermatozoa.