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Metabolic Inhibitor(s) in Orchardgrass (Dactylis glomerata L.)¹

Department of Animal Husbandry, University of Missouri, Columbia

ABSTRACT

Several solvents were used to prepare extracts and residues from orchardgrass (Dactylis glomerata L.) previously shown to cause poor animal performance. Both extracts and residues were tested for the presence of factors affecting cellulose digestion [metabolic inhibitor(s)] with the in vitro rumen fermentation technique developed at this station. Active extracts which caused an 8.5% reduction in the cellulose digestibility of a standard alfalfa were prepared from orchardgrass extracted with hot detergent (0.1% Tween 80), hot acid-detergent (0.1% Tween in 0.1 N HCl), and an azeotropic mixture of methanol and acetone. The results indicate the presence of more than one metabolic inhibitor in the orchardgrass used.

When a cellulose digestion permitting factor soluble in dilute acid and precipitated at pH 7.0 was removed from orchardgrass, the digestibility of the residue was reduced about 50%.

¹ Contribution from the Missouri Agricultural Experiment Station. Journal Series no. 5301. Approved by the Director.

² Present address: Department of Animal Science, Oklahoma State University, Stillwater.