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Method for Measurement of Gastrointestinal Absorption in Normal Animals, Combining Portal-Carotid Differences and Telemetered Portal Flow by Doppler Shift^{1, 2,}

Department of Animal Sciences, University of Kentucky, Lexington

ABSTRACT

The Doppler Ultrasonic Blood Flow System was used to measure the portal blood flow rate (mean 40.9 ml/min/kg) in seven calves ranging from 3 to 22 weeks of age, fed milk at 5% of body weight twice daily. Absorption estimates of total sugars and glucose were made by combining portal flow and portal-carotid blood concentration differences. Telemetry of blood flow information permitted measurements on unattended animals in a normal environment. Mean velocity of portal flow one hour postprandial was increased 9.7% by feeding. Net absorption of total sugars and glucose determined from plots of portal-carotid concentration differences over the 12-hour feeding interval, combined with the portal flow rate, accounted for 60% (calculated as glucose and galactose) of the lactose intake. Glucose alone accounted for 41% of the lactose intake. Difference between the total sugars and glucose absorbed indicates that some galactose or lactose per se is absorbed. Data also indicate that 20 to 40% of the fed lactose is not accounted for as absorbed sugar, suggesting utilization in the gut wall. The method developed, using Doppler shift principle to measure blood flow and portal-carotid concentration differences, is suitable for obtaining quantitative determinations of net absorption of nutrients from the gastrointestinal tract of ruminants in a normal environment.

¹ The data in this paper are from the dissertation of the senior author, submitted to the Graduate School of the University of Kentucky in partial fulfillment of the requirements for the Ph.D. degree.

² Published with the approval of the Director of the Kentucky Agricultural Experiment Station as Journal Article no. 67-5-75.

³ Present address: Department of Biochemistry and Nutrition, Virginia Polytechnic Institute, Blacksburg, Virginia.

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