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Relationship between California Mastitis Test Reaction and Bacteriological Analyses of Stripping Samples^{1, 2,}

Department of Animal Sciences, Washington State University, Pullman

ABSTRACT

Quarter stripping samples were examined to compare the relationship between the degree of inflammation as indicated by the leucocyte count or the California Mastitis Test reaction and the types and number of bacteria found in the sample.

Both pathogenic and nonpathogenic isolations were made on 1,036 quarter milk samples. The percentage of quarters from which a pathogen or nonpathogen was isolated was determined for each individual test reaction. At least one type of potential pathogen was found in 6.0, 6.5, 27.3, 64.7, and 71.3% of the quarters showing negative, trace, one, two, and three mastitis test reactions, respectively. The nonpathogens were found more frequently in quarters with low mastitis test reactions (negative, trace, and one) than in quarters with high mastitis test reactions (two and three). Thus, there appears to be a relationship between the California Mastitis Test reaction of a particular quarter and the types of bacteria isolated from that quarter.

Comparisons also were made between bacterial counts and leucocyte counts. A general relationship appeared to exist, but only a small portion of the leucocyte variation was associated with variation in bacterial numbers.

¹ College of Agriculture Scientific Paper no. 3050. Work conducted under Project 1680. This investigation was supported in part by grants from Johnson & Johnson and Washington State Dairy Products Commission.

² Data in this paper are from a thesis submitted by the senior author in partial fulfillment of the requirements for the degree of Master of Science.

³ Present address: Department of Dairy Science, University of Wisconsin, Madison.

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