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Comparison of Counts Using a Breed-Type Smear and Millipore Membrane Methods on Fresh and Preserved Milk Samples

Departments of Dairy Science and Mathematics and Statistics, University of Guelph, Ontario, Canada

ABSTRACT

Bacteria and somatic cell counts made with the direct microscopic count and millipore membrane count on thirty fresh and on thirty milk samples preserved with one tablet of Lactab (12 mg HgCl₂ and 52 mg K₂Cr₂O₇), Thomson and Copper Ltd., Liverpool, England, per 50 ml milk, were compared. The samples were classified in three groups of ten according to arbitrarily chosen somatic cell levels of <360,000; 360,000 to 900,000, and >900,000 cells per ml. The lowest cell level yielded the greatest average count variance. The within somatic cell level count variance was not affected significantly by milk treatment and method. The average somatic cell counts were higher for the millipore membrane method. Although they reacted the same under both milk treatments, use of preservative resulted in higher counts with greater influence on the millipore membrane method. Therefore, the millipore membrane count is a better method for determining somatic cells; significantly lower bacterial counts were obtained with it. The preservative had a marked adverse effect upon the stainability of bacteria independent of the method.