HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Henning, D. R. Articles by Elliker, P. R. Search for Related Content PubMed PubMed Citation Articles by Henning, D. R. Articles by Elliker, P. R.

Chemical Composition of a Bacteriophage for Streptococcus diacetilactis^{1, 2,}

Department of Microbiology, Oregon State University, Corvallis

ABSTRACT

Electron photomicrographs revealed that the tadpole-shaped phage particle had a head width and length of 60 mµ and a tail length of about 170 mµ. The dried phage contained 4.45% P and 12.8% of the protein was N.

Bacteriophages prepared in broth medium were recovered by differential centrifugation and purified in a cesium chloride density gradient. An amino acid analysis revealed the following mole percentages for these acids: lysine, 8.1; histidine, 1.7; arginine, 3.8; aspartic acid plus asparagine, 10.0; threonine, 7.5; serine, 6.8; glutamic acid plus glutamine, 12.0; proline, 3.4; glycine, 9.0; alanine, 9.5; valine, 8.9; methionine, 1.1; isoleucine, 7.9; tyrosine, 2.2 and phenylalanine, 2.8.

The nucleic acid of the phage was double-stranded DNA with a thermal melting point (Tm) of 84.3 C; from this, an average composition of 35.7% guanine plus cytosine was calculated.

¹ Data taken from a thesis by D. R. Henning in partial fulfillment of the requirements for the Ph.D. degree, Oregon State University, Corvallis, June, 1967.

² Technical Paper no. 2361, Oregon Agricultural Experiment Station.

³ Present address: U.S. Army Medical Research Unit, Letterman General Hospital, San F0rancisco, California.