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Glutaraldehyde Fixation of Casein Micelles for Electron Microscopy

Eastern Regional Research Laboratory¹ Philadelphia, Pennsylvania

ABSTRACT

Fixation of casein micelles from skimmilk (Bos taurus) for electron microscopy has been compared using osmium tetroxide, formaldehyde and, for the first time, glutaraldehyde. With the latter, a rapid procedure, coupled with a dispersion method, has been developed. Glutaraldehyde appears to preserve micelle structure better than either osmium tetroxide or formaldehyde and eliminates the need for time-consuming centrifugation. The method is easily adaptable to research on concentrated milks and artificial milk systems as well as on fresh skimmilk.

Glutaraldehyde-fixed micelles show a range of sizes from 500 to 2,500 Å. Most of the micelles fall in the range of 1,000 to 1,700 A in diameter, although this range will vary from milk to milk.

¹ Eastern Utilization Research and Development Division, Agricultural Research Service, U.S. Department of Agriculture.