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Occurrence of -Tocopherol and Variation of - and -Tocopherol in Bovine Milk Fat

Department of Agricultural Chemistry, University of Tokyo, Tokyo, Japan

ABSTRACT

It was confirmed that -tocopherol in addition to -tocopherol naturally occurred in milk fat as a normal component. -Tocopherol fractionated from milk fat was identified by thin-layer and gas-liquid chromatography, ultraviolet and infrared absorption spectra, and coupling reaction. This component was found in all samples of mixed milk and individual milk analyzed. The mean values of - and -tocopherol content in mixed milk estimated by the thin-layer chromatographic method for a year were 28.3 (range, 17.0–39.3) and 1.5 (range, 0.5–2.9) µg per gram of fat, respectively. Throughout seasons, -tocopherol varied from 29.2 to 39.3 (mean, 33.8) in summer (May–October), and from 17.0 to 28.0 (mean, 21.6) µg per gram of fat in winter (November–April). -Tocopherol varied from 1.0 to 2.5 (mean, 1.8) in summer and from 0.3 to 2.9 (mean, 1.1) µg per gram of fat in winter. Tocopherols other than - and -tocopherol were not detected in any samples analyzed.