HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Whiting, F. M. Articles by Stull, J. W. Search for Related Content PubMed PubMed Citation Articles by Whiting, F. M. Articles by Stull, J. W.

Detoxification of DDT by the Perfused Bovine Liver ^{1, 2,}

Department of Dairy Science, The University of Arizona, Tucson

ABSTRACT

Five bovine livers were isolated and perfused with DDT [2,2-bis (p-chlorophenyl)-1,1,1-trichloroethane] to study the ability of liver to detoxify chlorinated hydrocarbons. A perfusion apparatus was designed and constructed so as to minimize contact of perfusate or tissue vrith metal or plastic.

Within fifteen minutes after dosing there was a measurable decrease of DDT in the perfusate and a significant increase in DDD [2,2-bis (p-chlorophenyl) 1,1-dichloroethane]. Levels of DDE (2,2-bis (p-chlorophenyl) 1,1-dichloroethylene] were low at the beginning and increased only slightly during the experiment. It was demonstrated that isolated perfused bovine liver was capable of dehalogenating DDT to its less toxic analog, DDD. Production of DDE was probably due to catalytic action caused by hemoglobin breakdown. At the end of each two-hour perfusion experiment the livers still had a natural appearance and probably could have been used longer had it been necessary.

¹ Arizona Agricultural Experiment Station Technical Paper No. 1349.

² This work was supported in part by Grant no. EF-00627-02 from the USP Health Service and a grant from the United Dairymen of Arizona.