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Turbidimetric Behavior of Casein Fractions after Treatment with Rennet¹

Department of Animal Sciences, Washington State University, Pullman

ABSTRACT

Pure -casein solutions in concentrations of less than 0.1% produce measurable turbidities in acetate buffer in the presence of dilute rennet solutions at pH 5.8 and 30 C. In mixtures of _s- and -casein containing less than 20% - the increase in turbidity after 5 min is proportional to the percentage -casein in the mixture. Calcium is needed in the reaction mixture (.008M) to bring about the turbidity increases. The turbidity of pure _s-casein is not affected by addition of rennet. Variable increases in turbidity after rennet action on purified ß-casein fraction point to the difficulty of separation of -casein from ß-casein. Isolated whole casein reacts more slowly with rennet than does purifled -casein.

¹ Scientific Paper No. 3112. College of Agriculture Research Center Project No. 1809.