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Journal of Dairy Science Vol. 51 No. 1 16-21
© 1968 by American Dairy Science Association ®
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Host-Range Studies of Lactic Streptococcal Bacteriophages1, 2,

D. R. Henning3, C. H. Black4, W. E. Sandine and P. R. Elliker

Department of Microbiology, Oregon State University, Corvallis

ABSTRACT

The lytic patterns of 60 bacteriophage races toward 100 single lactic streptococcal strains allowed the establishment of eight phage groups, A through H. The phages within a particular group were generally species-specific. However, several exceptions were noted. Group A phages attacked primarily strains of S. diacetilactis. Groups B through H phages mainly attacked strains of S. lactis and S. cremoris. Groups G and H consisted of one phage that attacked only the homologous host. These host-range data were employed for selection of strains to be used in mixed-strain lactic starter cultures. A computer was used to identify strains which were suitable for composing mixed-strain cultures to provide maximum protection against phage. The strains selected by the computer were arranged in a sequence or rotation system suitable for use in initiating dairy fermentations. Protection from phage infection, as determined by a resistance index, remained high for six successive cultures, but the addition of the seventh culture to the rotation system increased the likelihood of phage attack.


FOOTNOTES

1 Data taken from a thesis by D. R. Henning in partial fulfillment of the requirements for the Ph.D. degree, Oregon State University, June, 1967.

2 Technical Paper no. 2338, Oregon Agricultural Experiment Station.

3 Present address: U.S. Army Medical Research Unit, Letterman General Hospital, San Francisco, California.

4 Present address: Department of Mechanical Engineering, Oregon State University.







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Copyright © 1968 by the American Dairy Science Association ®.