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Journal of Dairy Science Vol. 50 No. 9 1475-1480
© 1967 by American Dairy Science Association ®
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Influence of Light on Nuclear Size and Deoxyribonucleic Acid Content of Stored Bovine Spermatozoa

S. K. Paufler1 and R. H. Foote

Department of Animal Science, Cornell University, Ithaca, New York

ABSTRACT

Feulgen-positive material, assumed to be deoxyribonucleic acid (DNA), was determined by microspectrophotometry for 790 spermatozoa from 17 bulls stored in Cornell University Extender (CUE) at 5 C in sealed ampules. Factorially arranged treatments included light and dark, air and N2 gassing, and addition of ß-mercaptoethylamine (MEA). Light was associated with reduced motility and increased nuclear area, the correlation between the two variables being –0.51 (P < .05). Light also reduced the DNA content from 4.52 to 3.70 relative units (P < .01). The correlation between motility and total DNA content corrected for nuclear area was 0.70 (P < .01). N2 was more effective in preserving sperm motility than in preventing a decrease in DNA in sperm exposed to light. Bulls differed in size of the sperm nuclei (range 24.9 to 31.5 µ2), and in DNA content, but there was no significant relationship between either variable and fertility. Addition of 0.5 M MEA was toxic to spermatozoa and caused complete disappearance of the nuclei exposed to light in an air atmosphere. In N2 many nuclei were shrunken. Other nuclei in the presence of MEA were swollen or ruptured, or both, as were the nuclei of spermatozoa stored in the dark with air or N2.

FOOTNOTES

1 Present address: Tierärztliches Institut der Universität Göttingen, 3400 Göttingen, West Germany.






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Copyright © 1967 by the American Dairy Science Association ®.