HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Bezkorovainy, A. Search for Related Content PubMed PubMed Citation Articles by Bezkorovainy, A.

Physical and Chemical Properties of Bovine Milk and Colostrum Whey M-1 Glycoproteins.¹

Department of Biochemistry, Presbyterian—St. Luke's Hospital and University of Illinois College of Medicine, Chicago

ABSTRACT

The M-1 acidic glycoprotein fractions isolated from individual samples of bovine colostrum and milk whey, although homogeneous by ultracentrifugal and boundary electrophoresis criteria, contained several N-terminal amino acids and, in some instances, several bands on gel electropherograms and, thus, probably consisted of a series of closely related molecular species. Their average molecular weights were near 10,000, with high ß-values and negative specific rotations, indicating highly disorganized structures. Amino acid analysis showed high percentages of glutamic acid, proline, threonine, and isoleucine, and unusually low amounts of basic amino acids. Monosaccharides in the colostrum M-1 glycoprotein fractions were galactose, glucosamine, galactosamine, and sialic acid, the latter being easily removable by viral neuraminidase. The only hexose detected in the milk M-1 glycoprotein fraction was galactose.

¹ Supported by USPHS Grant GM-11985. Part of this paper was presented at the 152nd meeting of the American Chemical Society.