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Journal of Dairy Science Vol. 50 No. 8 1338-1340
© 1967 by American Dairy Science Association ®
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Factors Prolonging Survival of Unfrozen Bovine Spermatozoa1

R. H. Foote

Department of Animal Science, Cornell University, Ithaca, N.Y.

ABSTRACT

Bovine spermatozoa were extended with Cornell University Extender (CUE) and other buffered yolk extenders, placed in glass ampules and sealed, or gassed with various combinations of air, O2, CO2, and N2 before sealing. Inclusion of catalase was highly beneficial to spermatozoa stored in air or a CO2-O2 atmosphere. Sperm cell survival in CUE containing catalase with air in the gas phase was equal to survival observed in the presence of any gases used. Up to 42% of the spermatozoa were motile after 30 days of storage at 5 C.

FOOTNOTES

1 Partially supported by a grant from Eastern Artificial Insemination Cooperative, Inc.






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