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Distribution of Lactic Dehydrogenase and Transaminase in the Genital Tissues of Holstein-Friesian Bulls¹

Department of Animal Sciences, University of Arkansas, Fayetteville

ABSTRACT

The determination of LDH, LDH-1, LDH-5, GO-T, and GP-T has been performed in the various reproductive organs from five healthy young Holstein-Friesian bulls. No pathological ejaculates were obtained at any time during this investigation. The LDH activity in the various male reproductive organs was significantly (P < 0.01) higher than LDH-1 and LDH-5. Further, LDH-5 was significantly (P < 0.05) higher than LDH-1. Significant difference did exist in the activity of LDH-1 and LDH-5 among the various reproductive organs. The activity of GO-T observed in the various male reproductive organs was significantly (P < 0.01) higher than GP-T activity. Glutamic-oxalacetic transaminase was located primarily in the testis, ampulla, seminal vesicle, disseminate prostate, and bulbourethral gland. Also, the testis, corpus epididymidis, and disseminate prostate were the primary sites of GP-T activity in the male reproductive organs.

¹ This investigation was supported by U. S. Public Henlth Service Research Grant GM10654-03 from the National Institutes of Health. Published with the approval of the Arkansas Agricultural Experiment Station.

² Present address: Department of Dairy Science, Louisiana State University, Baton Rouge, Louisiana.