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Liver Vitamin B₁₂ Status of the Lactating Dairy Cow

Department of Animal Science, Cornell University, Ithaca, New York

ABSTRACT

Liver homogenates, centrifuged to remove nuclei and cell debris, were assayed for vitamin B₁₂ activity. Forty-one samples from 31 dairy cows were included in the study. Vitamin B₁₂ values ranged from 2.8 to 6.6 µg per g of protein. A significant (P < .05) part of the variation was accounted for by the multiple regression of liver vitamin B₁₂ on days fresh, milk production to date during lactation, daily milk yield, and the interactions of these variables. Of seven cows sampled during early lactation, and again at variable intervals but within the first 180 days of lactation, five showed a higher liver vitamin B₁₂ level at the second sampling. Liver and blood vitamin B₁₂ levels were poorly correlated in the dairy cow.

¹ Present address: Department of Dairy, Michigan State University, East Lansing, Michigan.

² Present address: U. S. Army Medical Research and Nutrition Laboratory, Fitzsimons General Hospital, Denver, Colorado.

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