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Journal of Dairy Science Vol. 50 No. 8 1273-1279
© 1967 by American Dairy Science Association ®
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Synthesis of D(—)ß-Hydroxybutyric Acid-14C

D. S. Sachan1 and C. L. Davis

Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

D(—)ß-hydroxybutyric acid-14C was synthesized from 14CO2 and acetate-1-14C using Hydrogenomonas H16. The labeled substrate is first incorporated into poly-ß-hydroxybutyric acid, which is easily isolated, purified, and degraded to D(—)ß-hydroxybutyric acid. Using acetate-1-14C as substrate, the overall conversion of substrate to the desired product is approxmately 60%. This method affords a simple, inexpensive means of synthesizing D(—)ß-hydroxybutyrie acid labeled with carbon-14 in specific positions of the molecule. It is equally adaptable to the synthesis of 14C-crotonic acid (trans isomer).

FOOTNOTES

1 These data were taken from a thesis submitted by the senior author to the Graduate College, University of Illinois, as a, partial requirement for the Master of Science degree in Dairy Science.






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Copyright © 1967 by the American Dairy Science Association ®.