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Identification of Yellow Material Remaining on Disks After Filtration of Milk¹

Animal Science Department, North Carolina State University, Raleigh

ABSTRACT

Cole et al. (2, 3) were the first to report that the yellow material appearing on milk sediment-test filter disks was associated with the mastitis condition. Choi and Forster (1) reported a significant relationship among degree of yellow color on milk sediment test filter disks and catalase values, CMT scores, direct microscopic leucocyte count, and A-esterase classes of the same milk samples. Milk carotene content has been shown to increase threefold after interrupted milking (6). Moreover, the greatest fraction of the carotenoids in milk is known to be ß-carotene (4). These observations suggested that the yellow color observed by others (1–3) from filtered milk was possibly ß-carotene. The purpose of the present study was to identify the yellow material from milk found on disks after filtration.

Methods. Holstein cows from the North Carolina State Experiment Station Herd were used, since milk from this breed normally contains the least amount of carotene (4).

¹ Contribution from the Animal Science Department, North Carolina Agricultural Experiment Station, Raleigh. Published with the approval of the Director of Research as paper no. 2365 of the Journal Series.