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Relationship of Ion Concentration to the Critical Temperatures During Freezing of Bull Spermatozoa

Department of Animal Science, Cornell University, Ithaca, New York

ABSTRACT

The over-all osmotic pressure of the extender is an important factor affecting the critical temperatures during which most of the damage to motility of bull spermatozoa occurs during freezing. For a 0.10 M Tris-yolk extender containing various levels of NaCl, KCl, or CaCl₂ the critical temperature begins at –30 to –35 C, and for a 0.30 M Tris solution containing corresponding salts this temperature begins at –10 to –15 C.

¹ Supported by a government fellowship from the United Arab Republic.

² Present address: Animal Production Department, College of Agriculture, Alexandria University, Alexandria, UAR.