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Effect of Ion Exchange Resins on Composition of Milk and Its Fractions

Milk and Food Research, National Center for Urban and Industrial Health, U. S. Public Health Service, Cincinnati, Ohio

ABSTRACT

Ultracentrifugation and dialysis studies were made to determine the distribution of calcium, phosphorus, and nitrogen in milk treated with ion exchange resins to remove radionuclides. In System I, milk was passed through Dowex 2-X8 (Cl:P:Cit) resin, acidified to pH 5.35 with citric acid, passed through IR-120 (Ca:Mg:K:Na) resin, and neutralized to pH 6.8 with KOH. In System II, the same cycle was followed, except that HCl was used to acidify milk and the milk was neutralized with IRA-68 (P:Cit:OH) weak base anionic resin.

A comparison of the test results for treated samples and those for the control sample showed increased concentrations in the casein nitrogen and in acid soluble phosphorus in serum, and a decreased amount of sedimented casein. The amount of calcium and acid soluble phosphorus associated with the sedimented caseinates also decreased. The extent of these alterations depended upon the type of acid used to acidify the milk. The effect of these changes in the ionic balance on the coagulation of milk by rennin and ethanol is briefly discussed.