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A Microbial-Free Milk Dialysate¹

Department of Dairy Technology, The Ohio State University, Columbus

ABSTRACT

Milk dialysate obtained by dialysis of water against milk and representing the colloidal-free fraction of milk, is used extensively as a native, protein-free milk buffer in protein research (3). Conventionally prepared dialysate from raw skimmilk will contain considerable numbers of bacteria, which decrease its keeping quality and research value.

This paper reports a method for preparing a dialysate from unheated raw skimmilk which has extended keeping quality, is apparently free of bacterial contamination, and does not require preservatives.

Approximately 250 ml of distilled water was placed in a 25-cm length of the dialysis bag (Visking casing, 36 mm), the ends of the bag sealed by knotting the tubing, and the bag and its contents immersed completely in distilled water and autoclaved at 15 psi for 1 hr.

Fresh whole milk, preferably of low bacterial count, was placed in sterile centrifuge bottles and subjected to low-temperature (5 C) centrifugation at 6,500 g for 10 min.

¹ Article no. 1: 67. Department of Dairy Technology. This investigation was supported by Public Health Service Grant EF-00180 from the Division of Environmental Health and Food Protection.