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Effect of Cold Shock and Freezing on Loss of Lipid from Spermatozoa¹

Department of Animal Industries, University of Connecticut, Storrs

ABSTRACT

Semen from bulls and boars was divided into three fractions and treated as follows: control, cold shocked, and frozen. There was a loss of lipid from bovine spermatozoa that had been cold shocked (P < 0.05) or frozen (P < 0.01) compared to the controls. This loss was aeompanied by a significant increase in lipid in seminal plasma of the corresponding fractions treated prior to separation from the cells. There were no significant changes in lipid or dry weight (DW) of porcine spermatozoa due to treatment.

¹ Scientific contribution no. 209, Agricultural Experiment Station, University of Connecticut, Storrs. This investigation was supported in part by Public Health Service Research Grant GM 08738 from the National Institutes of Health, Division of General Medical Sciences.

² Present address: Animal Reproduction Laboratory, Colorado State University, Fort Collins.