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Journal of Dairy Science Vol. 50 No. 3 307-312
© 1967 by American Dairy Science Association ®
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Distribution of Lipase in Milk Proteins. II. Dissociation from {kappa}-Casein with Dimethylformamide1

P. F. Fox, M. Yaguchi2 and N. P. Tarassuk

Department of Food Science and Technology, University of California, Davis

ABSTRACT

The distribution of lipase among milk proteins was investigated by chromatography on DEAE cellulose and filtration through Sephadex G-200. Protein components were identified by electrophoresis in starch gel containing 7 M urea. The effect of the dissociating agent dimethylformamide on distribution of lipase was studied. Results showed that the lipase in milk is normally associated with {kappa}-casein but can be dissociated from it. It is concluded that lipase is a discrete minor component of the casein system.

FOOTNOTES

2 Present address: Division of Biosciences, National Research Council, Ottawa, Canada.






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Copyright © 1967 by the American Dairy Science Association ®.