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Journal of Dairy Science Vol. 50 No. 3 305-306
© 1967 by American Dairy Science Association ®
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Centrifugal Sephadex Procedure for Fractionation of Concentrated Skimmilk, Whey, and Similar Biological Systems1

C. V. Morr, M. A. Nielsen and S. T. Coulter

Department of Food Science and Industries, University of Minnesota, St. Paul

ABSTRACT

A centrifugal Sephadex procedure has been adapted for separation of concentrated skimmilk, whey, and similar biological systems on the basis of molecular size. Concentrated skimmilk and whey were separated into two fractions: a) proteins and colloidal salts and b) lactose, salts, and other dissolved components.

This procedure permits rapid fractionation of relatively large volumes of viscous solutions (v <= 1/2 bed volume) with a minimum dilution of the protein fraction.

FOOTNOTES

1 Scientitic Journal Series Paper no. 6081, Minnesota Agricultural Experiment Station.






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Copyright © 1967 by the American Dairy Science Association ®.