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Journal of Dairy Science Vol. 50 No. 12 1983-1984
© 1967 by American Dairy Science Association ®
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Photometric Determination of Carbonyl Compounds1

G. A. Muck2, N. R. Sundararajan, J. Tobias and R. McL. Whitney

Department of Food Science, University of Illinois, Urbana

ABSTRACT

Henick et al. (2) proposed a method for the analysis of carbonyl compounds which was modified by Ganguly (1). However, certain additional modifications in the procedure were found to improve its usefulness.

Analytical Method

Reagents

(a) Carbonyl-free benzene: 5 g 2,4-dinitrophenylhydrazine (DNPH) and 1 g trichloroacetic acid (TCA) are added per liter of benzene. The mixture is refluxed for 1 hr and distilled through a Vigreux column. The first and last 50-ml portions are discarded.
(b) 4.3% (w/v) TCA in carbonyl-free benzene (TCA solution)
(c) 0.05% (w/v) DNPH in carbonyl-free benzene (DNPH solution)
(d) 80% (v/v) aqueous ethanol (80% ethanol)
(e) 4% (w/v) KOH(CP) in 80% ethanol (alc. KOH)
This solution is aged for at least ten days.
(f) Heptaldehyde: obtained from Eastman Organic Chemicals.
(g) K2Cr2O7 solution.
(h) Heptaldehyde 2,4-dinitrophenylhydrazone (heptaldehyde DNPH): Prepared by the method of Shriner et al. (7). After recrystallization, it is retained on a sintered glass filter and washed free of hydrazine by the method of Iddles et al. (3).

FOOTNOTES

1 A report of work done under contract with the U.S. Department of Agriculture and authorized by the Research and Marketing Act of 1946. The contract is being supervised by the Eastern Utilization Research and Development Division of the Agricultural Research Service.

2 Present address: Dean Foods Company, Rockford, Illinois.






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