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Metabolism of Bovine Semen. XVI. Pathways for Arginine Utilization¹

Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

The conversion of arginine to urea and other constituents of the urea cycle has been demonstrated in intact washed bovine spermatozoa. An alternate pathway for arginine degradation, resulting in guanido-butyrate and, subsequently, urea and -aminobutyrate, has also been discussed. Succinate, which would arise as a transamination product, has been identified as well as aspartic and glutamic acid, thereby implicating the citric acid cycle. Carbon dioxide evolution has been defined as having two different sources: the first, which is cyanide-sensitive, is apparently from the aliphatic chain; the second, which is less sensitive to inhibitors, arises from the guanido group. A compensatory mechanism was shown to exist when cyanide and nitrogen were used to inhibit the oxidative catabolism of arginine. This involved greater production of proline, allowing degradation to glutamate. Three possible pathways for the formation of proline were discussed in view of the intermediates identified.

¹ Authorized for publication on May 29, 1967, as paper no. 3267 in the journal series of the Pennsylvania Agricultural Experiment Station. Supported in part by Public Health Service Research Grant no. HD 00039-02.