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Journal of Dairy Science Vol. 50 No. 11 1831-1838
© 1967 by American Dairy Science Association ®
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Estradiol Metabolism and Its Inhibition in Bull Semen1–3,

Ralph R. Hathaway

Department of Molecular and Genetic Biology, University of Utah, Salt Lake City

ABSTRACT

Bull semen was examined to determine if estradiol and testosterone were oxidized. Spermatozoa converted 14C-17ß-estradiol into a single product identified as estrone. The reverse reaction did not take place, nor was testosterone metabolized by the sperm. The reaction with 17ß-estradiol was completely inhibited by seminal plasma, so that spermatozoa had to be washed and resuspended in Ringer's solution for the conversion to take place. The inhibition was not due to a heat-labile constituent of seminal plasma nor, apparently, to pyridine nucleotide nucleosidase in semen. Added cofactors (NAD or NADP) did not enhance the reaction in whole sperm, but did restore activity to sonified sperm. Dilute suspensions of spermatozoa were several times more active than dense suspensions. Ejaculates collected from the same bull over a 14-month period showed wide variations in ability to convert 17ß-estradiol, with complete lack of activity occurring in some specimens.

FOOTNOTES

1 This paper is dedicated to my friend and fellow scientist, Dr. Albert Tyler, on the occasion of his sixtieth birthday.

2 This work was supported by Grants HD 01481 and AM 06730 from U. S. Public Health Service.

3 The trivial names used in this paper are: Androstenedione ({Delta}4-androstene-3,17-dione); pregnandiol (5 ß-pregnane-3{alpha}, 20{alpha}-diol); dehydroepi-androsterone ({Delta}-androsten-3ß-ol-17-one); 17ß-estradiol, estradiol, estrone, testosterone, and 11-deoxycorticosterone.






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Copyright © 1967 by the American Dairy Science Association ®.