HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Katz, I. Articles by Keeney, M. Search for Related Content PubMed Articles by Katz, I. Articles by Keeney, M.

Rapid Method for Isolation of Unesterified Sterols and Its Application to Detection of Milk Fat Adulteration with Vegetable Oils¹

Department of Dairy Science, University of Maryland, College Park

ABSTRACT

Unesterified sterols were isolated from 900-mg samples of fat by using a column of digitonin impregnated on Celite 545, Schwartz et al. (4), then eluting the sterols with dimethylsulfoxide. The sterols were extracted from the dimethylsulfoxide with hexane-benzene mixtures in a condition suitable for analysis by gas-liquid chromatography. The addition of 1% corn, cottonseed, soybean, or peanut oil could be easily detected by the presence of ß-sitosterol. Coconut and safflower oil adulteration were detected at the 2% level. Because of small amounts of ß-sitosterol present in commercial emulsifiers, ice cream fat adulterated with corn, cottonseed, soybean, or peanut oil would he detected at the 2 to 2.5% level, while coconut and safflower oil adulteration would be detected at the 4 to 5% level.

¹ Scientific Article no. A1361, Contribution no. 3940, of the Maryland Agricultural Experiment Station.

² Present address: International Flavors and Fragrances, 521 West 57th Street, New York, N. Y. 10019.