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Journal of Dairy Science Vol. 50 No. 10 1714-1716
© 1967 by American Dairy Science Association ®
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Incorporation of 15N-Ammonia into Blood Amino Acids and Nonprotein Nitrogen of Ruminants

A. Kosharov1, A. Bensadoun2, L. H. Breuer, Jr.3, J. K. Loosli, C. J. Morris and J. T. Reid

Animal Science Department and U. S. Plant, Soil and Nutrition Laboratory, USDA, Cornell University, Ithaca, New York

J. O. Legg

Soil and Water Conservation Research Division, USDA, Beltsville, Maryland

ABSTRACT

Nonprotein nitrogen is utilized by ruminal microorganisms in the synthesis of amino acids (6). Ammonia-N can also be used by animal tissues in the amidation of alpha-keto acids and in the amidation of glutanic acid (2). This study is concerned with the utilization of ammonia-N by ruminants, as indicated by the appearance of 15N in amino acids and other nitrogenous compounds of portal and carotid blood following the intraruminal administration of 15N-ammonium chloride.

Experimental Procedure

A mature Rambouillet x Hampshire cross wether fitted with a ruminal fistula was fed mixed hay (timothy-alfalfa) ad libitum and 0.45 kg of ground yellow corn containing 2 g of ammonium chloride per day for a four-week preliminary period. One week before the experimental day the portal vein of the wether was catheterized (1). On the day of the experiment, 2.0 g of 15NH4Cl containing 0.487 g of 15N was added quantitatively to the rumen contents through the rumen fistula, one hour after the animal was fed the daily ration of hay and ground corn without added NH4Cl.

FOOTNOTES

1 Present address: Physiology and Biochemistry, Moscow University, USSR.

2 Present address: Poultry Science Department, Cornell University, Ithaca, New York.

3 Present address: Animal Science Department, Texas A & M University, College Station, Texas.






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