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Department of Dairy Science, University of Illinois, Urbana
ABSTRACT
Two designed experiments were conducted to test the effect of freezing diluted bull semen to –79 to –88 C on spermatozoan aging, as indicated by a decrease with the passage of time in their fertility, accompanied by an increase in the incidence of embryonic and early fetal death of the zygotes. Experiment I was designed to test the influence of month of the year (season) on reproductive efficiency of cows, and Experiment II, the influence of season on bulls. At approximately 38 to 40 degrees north latitude, the bulls are responsible for the seasonal variations in fertility observed. Aging of frozen spermatozoa occurs more rapidly in summer-collected semen than in semen collected from November to April. The manifestations of spermatozoan aging appear more rapidly in frozen semen than anticipated from their occurrence after use of liquid semen and the assumption of a metabolic temperature coefficient of about 2.
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