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Factors Affecting Reliability of Catalase Test Results. I. Sanitizers¹

Department of Animal Sciences, University of Kentucky, Lexington

ABSTRACT

The influence of commercial chemical sanitizers in concentrations of 5 to 50 ppm on the catalase test was determined. Chlorine sanitizers caused a pronounced reduction in the percentage of oxygen produced. The effect increased with increasing concentrations of the sanitizer and was more pronounced when the test was made after 5 or 24 hr of storage at 4.5 C following addition of the sanitizers, than when tested immediately.

Iodophors, quaternary ammonium compounds, an acid sanitizer, and a sanitizer containing both potassium iodide and chloramine-T did not influence test results. These results indicate that chlorine sanitizers interfere with the catalase test. The reliability of the results of the test could be markedly reduced by contamination of the milk with these products, but contamination by other types of sanitizers would not alter the reliability of the test.

¹ The investigation reported in this paper (Journal Article no. 66-5-71) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director.