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Use of C¹⁴ to Study Utilization of Substrates in Ruminants

Department of Dairy Science, University of Idaho, Moscow

ABSTRACT

Carbon 14-labeled compounds are used to examine the utilization of substrates by ruminants in experiments where the following parameters are measured: 1) oxidation rates, 2) turnover rates, 3) body pool size of substrates, 4) production rates of rumen fermentation products, 5) transfer of C¹⁴ from labeled substrates to various other metabolic compounds. The C¹⁴-labeled substrates most commonly studied in ruminants are acetate, propionate, butyrate, and glucose. Recently, data on the metabolism of C¹⁴-labeled higher fatty acids and triglycerides in ruminants have appeared in the literature (5, 6, 11). This discussion will not be an extensive review of the literature on metabolism of C¹⁴-labeled substrates in ruminants, but will consider very briefly some of the techniques involved in and limitations of the use of C¹⁴ to study the utilization of some substrates in the intact ruminant.

Kinetics of the Body Pool of Various Substrates

Pool size and turnover rate. Two techniques have been used to study the size and turnover rate of the body glucose pool and the acetate pool.