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Journal of Dairy Science Vol. 49 No. 7 792-795
© 1966 by American Dairy Science Association ®
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DEAE-Cellulose-Urea Chromatography of Casein in the Presence of 2-Mercaptoethanol

M. P. Thompson

Eastern Regional Research Laboratory, Eastern Utilization Research and Development Division, USDA, Philadelphia, Pennsylvania

ABSTRACT

{kappa}-Casein, after reduction of the disulfide bond(s) by 2-inercaptoethanol, can be separated from {alpha}s1- and ß-casein variants, as well as from whole casein, by DEAE-cellulose-urea chromatography. When {kappa}-easein is in the reduced form it elutes from DEAE-cellulose at 0.05 m NaCl. Use of a reduction step simplifies purification of the major casein components ({alpha}s1-, ß) by chromatography.




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W. N. Marmer, P. Magidman, and H. M. Farrell JR
Pyrolysis Gas Chromatography of Wool: Part II: Detection and Quantitation of Tryptophan in Wool and Simple Proteins
Textile Research Journal, October 1, 1989; 59(10): 616 - 622.
[Abstract] [PDF]


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