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Effect of Sodium Hypochlorite on the Catalase Test¹

Department of Dairy Science, University of Kentucky, Lexington

ABSTRACT

The catalase test has been widely used as a screening procedure for detection of abnormalities in the milk supply (1, 4). It is one of five tests recommended for this purpose by the U. S. Public Health Service (6). Although considerable work (2, 3, 5) has been done on the effect of techniques and storage of the milk, there is a definite lack of information on factors which might influence the reliability of the catalase test results. As a part of a comprehensive study, the influence of sanitizers was investigated. Results obtained with sodium hypochlorite are presented in this paper.

Experimental Procedure

Samples of mixed-herd milk were obtained at the University of Kentucky dairy plant. A concentrated solution of sodium hypochlorite (prepared from a commercial liquid sodium hypochlorite sanitizer) was added to the milk to give concentrations of 10, 15, 25, and 50 ppm available chlorine. The catalase activity of the samples immediately after addition of chlorine and holding at 4.5 C for 5 hr, was determined by the inverted tube method described by Spencer and Simon (5). Graduated centrifuge tubes with one-hole rubber stoppers fitted with straight glass tubing were used (3).

¹ Published with the approval of the Director of the Kentucky Agricultural Experiment Station as Journal Article no. 66-6-27.