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Journal of Dairy Science Vol. 49 No. 6 631-635
© 1966 by American Dairy Science Association ®
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A-Esterase Activities of Milk From Cows with Experimentally Induced Mastitis1,2,

R. R. Marquardt3, T. L. Forster, G. R. Spencer and G. H. Stabenfeldt4

Department of Animal Sciences (Dairy) and Department of Veterinary Pathology, Washington State University, Pullman

ABSTRACT

Variations in CMT (California Mastitis Test), cell count, catalase, chloride, and A-esterase levels following the inoculation of normal lactating quarters of two cows with varying numbers of {alpha}ß hemolytic staphylococci (previously isolated from a cow with mastitis) are described. Since the organism did not produce as strong a response as desired, the quarters were reinfected three times during the 17-day experimental period.

Infusions of sterile broth into the two control quarters of Cows 506 and 583 did not affect A-esterase levels. Infusions of varying levels of {alpha}ß hemolytic staphylococci into the three remaining quarters of each of the two cows generally resulted in a marked increase in A-esterase activity within a 12-hr period, followed by a gradual decline. Similar patterns of change were also observed for catalase and cell counts. Coefficients of correlation between A-esterase and the four other tests were: A-esterase x CMT = 0.69, n = 192 individual samples tested; A-esterase x cell counts = 0.78, n = 188; A-esterase x catalase = 0.80, n = 192; and A-esterase x chloride = 0.60, n = 192.


FOOTNOTES

1 Scientific Paper no. 2756, College of Agriculture, Washington State University. Work was conducted under Projects 1023, 1523, and 1680.

2 Data from this paper are from a thesis submitted by the senior author in partial fulfillment of the requirements for the degree of Doctor of Philosophy.

3 Present address: Department of Chemistry, Washington State University, Pullman.

4 Present address: Department of Physiology, Oklahoma State University, Stillwater.







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