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Department of Animal Sciences (Dairy) and Department of Veterinary Pathology, Washington State University, Pullman
ABSTRACT
Variations in CMT (California Mastitis Test), cell count, catalase, chloride, and A-esterase levels following the inoculation of normal lactating quarters of two cows with varying numbers of
ß hemolytic staphylococci (previously isolated from a cow with mastitis) are described. Since the organism did not produce as strong a response as desired, the quarters were reinfected three times during the 17-day experimental period.
Infusions of sterile broth into the two control quarters of Cows 506 and 583 did not affect A-esterase levels. Infusions of varying levels of
ß hemolytic staphylococci into the three remaining quarters of each of the two cows generally resulted in a marked increase in A-esterase activity within a 12-hr period, followed by a gradual decline. Similar patterns of change were also observed for catalase and cell counts. Coefficients of correlation between A-esterase and the four other tests were: A-esterase x CMT = 0.69, n = 192 individual samples tested; A-esterase x cell counts = 0.78, n = 188; A-esterase x catalase = 0.80, n = 192; and A-esterase x chloride = 0.60, n = 192.
1 Scientific Paper no. 2756, College of Agriculture, Washington State University. Work was conducted under Projects 1023, 1523, and 1680.
2 Data from this paper are from a thesis submitted by the senior author in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
3 Present address: Department of Chemistry, Washington State University, Pullman.
4 Present address: Department of Physiology, Oklahoma State University, Stillwater.
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