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Journal of Dairy Science Vol. 49 No. 5 500-503
© 1966 by American Dairy Science Association ®
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Effect of Method of Semen Collection and Tranquilization on Semen Quality and Bull Behavior1

M. E. Wells, W. N. Philpot2, S. D. Musgrave, E. W. Jones and W. E. Brock

Department of Dairy Science and College of Veterinary Medicine Oklahoma State University, Stillwater

ABSTRACT

Three groups of Holstein bulls were used to determine the effects of repeated electroejaculation and tranquilization on semen quality and bull behavior. Group 1 was collected with the artificial vagina. Groups 2 and 3 were collected by electroejaculation, Group 3 being tranquilized prior to collection. Groups 2 and 3 were significantly higher than Group 1 (P < 0.01) in volume of semen per ejaculate and pH and significantly lower (P < 0.01) in number of live sperm per milliliter. Group 3 was significantly lower (P < 0.01) than Group 2 in pH and significantly higher (P < 0.01) in live sperm per milliliter, live sperm per ejaculate, and total sperm per milliliter. Group 2 was significantly lower (P < 0.05) than Group 1 in live sperm per ejaculate. Samples from Groups 1 and 3 lived significantly longer (P < 0.05) than samples from Group 2. No significant differences among groups were found for initial rate of movement, percentage of motile sperm, or per cent abnormal sperm. These results indicate that tranquilization aided in collection of higher-quality semen by electroejaculation.

Tranquilization was effective in reducing straining and tetany in bulls collected by electroejaculation. Tranquilized animals were consistently easier to electroejaculate. No serious side effects resulted from repeated tranquilization.

FOOTNOTES

1 Data presented herein were taken in part from a thesis submitted by the senior author to the faculty of the Graduate School, Oklahoma State University, in partial fulfillment of the degree. Doctor of Philosophy, May, 1962.

2 Present address: North Louisiana Hill Farm Experiment Station, Homer, Louisiana.






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