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Application of Various Acid Dyes for Estimation of Protein in Milk by Dye-Binding

Department of Agricultural Chemistry, The University of Tokyo, Japan

ABSTRACT

Twenty-three acid dyes which could be supplied in quantity and in rather pure form were selected, including Amido Black 10B and Orange G, and were compared for solubility in citrate buffer, stability of the solution, absorbance index, and binding capacity with protein in milk. Four dyes. Acid Orange II, Resorcine Brown, Xylene Acid Milling Orange 2G, and Diacid Light Green GS, excellent in binding capacity, were finally chosen, and field tests carried out for six months with individual Holstein and Jersey milks. From the results it became evident that Diacid Light Green GS was much superior, and Resorcine Brown most inferior for estimating protein. The three dyes, except Resorcine Brown, can be applied with safety for mass protein testing of milk. Amido Black 10B and Orange G appeared to be relatively inferior, both in protein binding and in desired properties of a dye.