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Preparation of Goat ß-Lactoglobulin

Eastern Regional Research Laboratory, Philadelphia, Pennsylvania³

ABSTRACT

Sen and Chaudhuri (11) reported the crystallization of goat ß-lactoglobulin from an isoelectric, salt-free solution by a modification of the method of Aschaffenburg and Drewry (1). Phillips and Jenness (8), however, were unsuccessful in their attempts to crystallize goat ß-lactoglobulin in a similar fashion. Askonas (2) also failed in an earlier effort. In all cases (2, 8, 11, 12), however, the protein was crystallized from solutions containing ammonium sulfate, as described by Askonas (2).

In the course of an investigation comparing the antigenicity of various ß-lactoglobulins, it became necessary to prepare goat ß-lactoglobulin from large amounts of pooled herd milk. The method employed is essentially that of Sen and Chaudhuri (11), except for two modifications which enabled us to prepare a large quantity of crystalline material in a relatively short time. The modifications precede the application of the Sen and Chaudhuri procedure (11). The first modification requires removal of the cream from goat's milk using a DeLaval cream separator;¹ the second requires the acid precipitation of casein at pH 4.2.²

³ Eastern Utilization Research and Development Division, Agricultural Research Service, U. S. Department of Agriculture.