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Storage of Lactic Streptococci. I. Effect of pH on Survival and Endogenous Metabolism in Phosphate Buffer¹

Department of Animal Industry and Veterinary Science and the Department of Plant Pathology, Bacteriology, and Entomology, West Virginia University, Morgantown

ABSTRACT

Washed cells of Streptococcus lactis E and Streptococcus cremoris 18 were stored up to ten days at 4 C in 0.05 M phosphate buffers in the range of pH 4.5–11.5. Decreased glycolytic activity of the recovered cells, when assayed in milk, was due to loss of viability. Viability was dependent upon length of storage and pH of the suspending buffer. The optimum pH for storage of these organisms was found to be 8.5. Analyses for amino nitrogen indicate that endogenous metabolism is required for maintenance of maximum viability during storage. When aged cells were recovered from the pH 4.5 buffer and resuspended at pH 8.5 for 24 hr, endogenous metabolism was stimulated and a portion of the glycolytic activity restored.

¹ Published with the approval of the Director of the West Virginia University Agricultural Experiment Station as Scientific Paper no. 859.