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Internal Structure of Casein Micelles from Bovine Milk

Division of Biosciences, National Research Council, Ottawa, Ontario, Canada

ABSTRACT

The internal structure of casein micelles from bovine milk has been reinvestigated. Observations from electron microscopy, X-ray diffraction, and enzymatic digestion of the micelles followed by centrifugation suggested that electron-dense particles in the micelles are probably granules of colloidal calcium phosphate and not protein aggregates. These granules are embedded in a surrounding matrix of caseins to form the micelle. The foci of formation of the calcium phosphate granules may be either the ester phosphate groups or the -amino groups of lysine.