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Lactic Streptococcal Phage-Associated Lysin. I. Lysis of Heterologous Lactic Streptococci by a Phage-Induced Lysin¹

Department of Agricultural Biochemistry, University of Vermont, Burlington

ABSTRACT

This investigation concerns the study of a lactic phage-associated lysin released when Streptococcus lactis strain C10 cells were lysed by their homologous c10 phage. The lysin appeared in the medium when the cells lysed. A spreading halo surrounding the clear phage plaques was observed after prolonged incubation of double agar layer plates. Both living and sensitized C10 cells were lysed by the c10-lysin. All heterologous lactic streptococcal strains studied thus far were lysed by the c10-lysin. A lysozyme-sensitive strain of Sarcina lutea was very resistant to c10-lysin. Micrococcus lysodeikticus substrate cells (Difco) were lysed rapidly during the initial reaction period, but lysis diminished rapidly soon thereafter. The role of c10-lysin in the nascent phenomenon is discussed.

¹ From a portion of a dissertation by the senior author in partial fulfillment of requirements for the Ph.D. degree. Contribution from the University of Vermont, Agricultural Experiment Station, Journal Article no. 164.